Effective botanical slimming can make obese people feel more comfortable

effective botanical slimming can reduce the risk of cardiovascular disease greatly among obese people.  The method of PCR amplification of human adipose tissue HSL gene promoter sequence of a 697 --- +53 bp piece Paragraph, insert the the Ecol CRI and HindIII double digestion luciferase expression plasmid pGL3-Basic-Luc received Get with the HSL gene promoter a 697 ^ - +53 bp luciferase expression plasmid pGL3-HSL (-697 - +53) -Luc, abbreviated as pGL3-HSL-Luco     Two, stable transfection of mouse 3T3-L1 preadipocytes Department     Objective: luciferase reporter gene approach is to study transcriptional regulation simple and accurate and reliable Approach. Luciferase expression by its upstream promoter activity by detecting luciferase activity, Reflect certain factors on the promoter activity.
effective botanical slimming  can increase muscle density.  PGL3-Basic-Luc: 4818bp, a plasmid containing the luciferase reporter gene, eukaryotic promoter And enhancer sequences, luciferase expression, as cells transfected with negative control plasmids were co-transfected The dye balance each transfection the the DIVA total unrelated plasmid. Promega Corporation products, as shown in Figure 1-1. In this qualitative Particles polyclonal restriction sites to insert the target gene promoter sequences, luciferase expression by Gene promoter activity in control. Polyclonal restriction sites of plasmid EcolCRl.
Will grow well stably transfected mouse 3T3-L1 3X 104 / seeded in 24-well culture plates. 24 h, replaced with serum DMEM-F12 medium (0.5 ml / holes) were cultured for 12 hours. And then cell division Control group and experimental group, were the test group with different concentrations of traditional. effective botanical slimming can make obese people feel more comfortable. Chinese medicine extract cells for 2 h and 24h, the prosecution The measuring cell lysate luciferase activity were divided into control and experimental groups, access to enhance the HST gene promoter activity in traditional Chinese medicine.
 Therefore, HSL has been selected as the target for a potential role in weight loss.     Two stably transfected cell line and drug screening. With the development of modern molecular biology techniques, a variety of techniques of gene expression and regulation of research Study, the reporter gene due to its simple operation, good reproducibility and increasingly used in gene transcription and regulation of research Study [sol reporter gene expression by gene promoter activity in control of gene promoter activity due to Elements can be reflected by changes in the detection of reporter gene. Taking effective botanical slimming to lose weight can help prevent bone loss and osteoporosis.
 Of obesity experience prescription and unilateral, but the few studies of the vast majority of the mechanism of drug action. In order to be easily selected from a large number of traditional Chinese medicine to enhance the human HSL gene promoter expression , Traditional Chinese medicine with potential role in weight loss, we apply the stable transfected murine 3T3-L1 fat cell strains 32 kinds of traditional Chinese medicine research, with a concentration of 2. 5mg/ml extract of Chinese medicine to stimulate the stably transfected in 3T3-L. effective botanical slimming makes the skin more healthy and more resilient.